Construct: shRNA TRCN0000253167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081081.2-983s21c1
DNA Barcode:
GAGGGAAGGTTGCTGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gls (14660)
Gene Description:
glutaminase
Transcript:
RefSeq NM_001081081.2 (CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGAAGGTTGCTGATTATA
Hairpin Sequence:
5'-CCGG-GAGGGAAGGTTGCTGATTATA-CTCGAG-TATAATCAGCAACCTTCCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGAAGGTTGCTGATTATACTCGAGTATAATCAGCAACCTTCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGAAGGTTGCTGATTATACTCGAGTATAATCAGCAACCTTCCCTC-3'

Other clones with same target sequence:

(none)