Construct: shRNA TRCN0000253223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081027.2-971s21c1
DNA Barcode:
ATCCAAGACTACAGGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnt2 (240776)
Gene Description:
potassium channel, subfamily T, member 2
Transcript:
RefSeq NM_001081027.2 (CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCAAGACTACAGGATTATT
Hairpin Sequence:
5'-CCGG-ATCCAAGACTACAGGATTATT-CTCGAG-AATAATCCTGTAGTCTTGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCAAGACTACAGGATTATTCTCGAGAATAATCCTGTAGTCTTGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCAAGACTACAGGATTATTCTCGAGAATAATCCTGTAGTCTTGGAT-3'

Other clones with same target sequence:

(none)