Construct: shRNA TRCN0000253240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029367.1-543s21c1
DNA Barcode:
AGGATATACTGCACTGATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spaca3 (75622)
Gene Description:
sperm acrosome associated 3
Transcript:
RefSeq NM_029367.1 (CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATATACTGCACTGATTTG
Hairpin Sequence:
5'-CCGG-AGGATATACTGCACTGATTTG-CTCGAG-CAAATCAGTGCAGTATATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATATACTGCACTGATTTGCTCGAGCAAATCAGTGCAGTATATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATATACTGCACTGATTTGCTCGAGCAAATCAGTGCAGTATATCCT-3'

Other clones with same target sequence:

(none)