Construct: shRNA TRCN0000253290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033320.2-1986s21c1
DNA Barcode:
CAGGCTCTGATGGTGACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Carmil2 (234695)
Gene Description:
capping protein regulator and myosin 1 linker 2
Transcript:
RefSeq NM_001033320.2 (CURRENT)
Match location:
Position 1986 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCTCTGATGGTGACATTA
Hairpin Sequence:
5'-CCGG-CAGGCTCTGATGGTGACATTA-CTCGAG-TAATGTCACCATCAGAGCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCTCTGATGGTGACATTACTCGAGTAATGTCACCATCAGAGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCTCTGATGGTGACATTACTCGAGTAATGTCACCATCAGAGCCTG-3'

Other clones with same target sequence:

(none)