Construct: shRNA TRCN0000253308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172771.2-7288s21c1
DNA Barcode:
CTTGCAGCCCACCCGTTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dmxl2 (235380)
Gene Description:
Dmx-like 2
Transcript:
RefSeq NM_172771.2 (CURRENT)
Match location:
Position 7288 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCAGCCCACCCGTTAAAT
Hairpin Sequence:
5'-CCGG-CTTGCAGCCCACCCGTTAAAT-CTCGAG-ATTTAACGGGTGGGCTGCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCAGCCCACCCGTTAAATCTCGAGATTTAACGGGTGGGCTGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCAGCCCACCCGTTAAATCTCGAGATTTAACGGGTGGGCTGCAAG-3'

Other clones with same target sequence:

(none)