Construct: shRNA TRCN0000253309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172771.2-5439s21c1
DNA Barcode:
CATGGTTATTGCCCGTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dmxl2 (235380)
Gene Description:
Dmx-like 2
Transcript:
RefSeq NM_172771.2 (CURRENT)
Match location:
Position 5439 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGTTATTGCCCGTTTATT
Hairpin Sequence:
5'-CCGG-CATGGTTATTGCCCGTTTATT-CTCGAG-AATAAACGGGCAATAACCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGTTATTGCCCGTTTATTCTCGAGAATAAACGGGCAATAACCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGTTATTGCCCGTTTATTCTCGAGAATAAACGGGCAATAACCATG-3'

Other clones with same target sequence:

(none)