Construct: shRNA TRCN0000253359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081337.1-5823s21c1
DNA Barcode:
GTTGCTGTAGAGACGTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sipa1l2 (244668)
Gene Description:
signal-induced proliferation-associated 1 like 2
Transcript:
RefSeq NM_001081337.1 (CURRENT)
Match location:
Position 5823 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGCTGTAGAGACGTATTTA
Hairpin Sequence:
5'-CCGG-GTTGCTGTAGAGACGTATTTA-CTCGAG-TAAATACGTCTCTACAGCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGCTGTAGAGACGTATTTACTCGAGTAAATACGTCTCTACAGCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGCTGTAGAGACGTATTTACTCGAGTAAATACGTCTCTACAGCAAC-3'

Other clones with same target sequence:

(none)