Construct: shRNA TRCN0000253382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001102430.1-2499s21c1
DNA Barcode:
GTTTCAGCTCTTCGTCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arfgef1 (211673)
Gene Description:
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Transcript:
RefSeq NM_001102430.1 (CURRENT)
Match location:
Position 2499 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCAGCTCTTCGTCTATTT
Hairpin Sequence:
5'-CCGG-GTTTCAGCTCTTCGTCTATTT-CTCGAG-AAATAGACGAAGAGCTGAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCAGCTCTTCGTCTATTTCTCGAGAAATAGACGAAGAGCTGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCAGCTCTTCGTCTATTTCTCGAGAAATAGACGAAGAGCTGAAAC-3'

Other clones with same target sequence:

(none)