Construct: shRNA TRCN0000253395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033226.2-1411s21c1
DNA Barcode:
GGGCCCTCGCTTAGCAAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Calr4 (108802)
Gene Description:
calreticulin 4
Transcript:
RefSeq NM_001033226.2 (NON-CURRENT)
Match location:
Position 1411 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCCCTCGCTTAGCAAATTA
Hairpin Sequence:
5'-CCGG-GGGCCCTCGCTTAGCAAATTA-CTCGAG-TAATTTGCTAAGCGAGGGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCCCTCGCTTAGCAAATTACTCGAGTAATTTGCTAAGCGAGGGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCCCTCGCTTAGCAAATTACTCGAGTAATTTGCTAAGCGAGGGCCC-3'

Other clones with same target sequence:

(none)