Construct: shRNA TRCN0000253407
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001033296.2-673s21c1
- DNA Barcode:
- CAAGCCAAGGCACTGATATAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sel1l2 (228684)
- Gene Description:
- sel-1 suppressor of lin-12-like 2 (C. elegans)
- Transcript:
- RefSeq NM_001033296.2 (CURRENT)
- Match location:
- Position 673 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000253407)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAAGCCAAGGCACTGATATAT
- Hairpin Sequence:
-
5'-CCGG-CAAGCCAAGGCACTGATATAT-CTCGAG-ATATATCAGTGCCTTGGCTTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAAGCCAAGGCACTGATATATCTCGAGATATATCAGTGCCTTGGCTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAAGCCAAGGCACTGATATATCTCGAGATATATCAGTGCCTTGGCTTG-3'
Other clones with same target sequence:
(none)
