Construct: shRNA TRCN0000253408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081046.1-438s21c1
DNA Barcode:
CAACTTGAAGCCTTCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab3 (70894)
Gene Description:
EF-hand calcium binding domain 3
Transcript:
RefSeq NM_001081046.1 (CURRENT)
Match location:
Position 438 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTGAAGCCTTCCGTAAT
Hairpin Sequence:
5'-CCGG-CAACTTGAAGCCTTCCGTAAT-CTCGAG-ATTACGGAAGGCTTCAAGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTGAAGCCTTCCGTAATCTCGAGATTACGGAAGGCTTCAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTGAAGCCTTCCGTAATCTCGAGATTACGGAAGGCTTCAAGTTG-3'

Other clones with same target sequence:

(none)