Construct: shRNA TRCN0000253469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027866.1-1083s21c1
DNA Barcode:
TCTGAATAGGTGGCGTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Colec11 (71693)
Gene Description:
collectin sub-family member 11
Transcript:
RefSeq NM_027866.1 (NON-CURRENT)
Match location:
Position 1083 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGAATAGGTGGCGTATTAT
Hairpin Sequence:
5'-CCGG-TCTGAATAGGTGGCGTATTAT-CTCGAG-ATAATACGCCACCTATTCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGAATAGGTGGCGTATTATCTCGAGATAATACGCCACCTATTCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGAATAGGTGGCGTATTATCTCGAGATAATACGCCACCTATTCAGA-3'

Other clones with same target sequence:

(none)