Construct: shRNA TRCN0000253478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033175.2-582s21c1
DNA Barcode:
TTGAGCTGTTGTACTACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cln6 (76524)
Gene Description:
ceroid-lipofuscinosis, neuronal 6
Transcript:
RefSeq NM_001033175.2 (CURRENT)
Match location:
Position 582 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGAGCTGTTGTACTACTATG
Hairpin Sequence:
5'-CCGG-TTGAGCTGTTGTACTACTATG-CTCGAG-CATAGTAGTACAACAGCTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGAGCTGTTGTACTACTATGCTCGAGCATAGTAGTACAACAGCTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGAGCTGTTGTACTACTATGCTCGAGCATAGTAGTACAACAGCTCAA-3'

Other clones with same target sequence:

(none)