Construct: shRNA TRCN0000253561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100177.1-126s21c1
DNA Barcode:
GGAGATATCATGTGCTATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Calcoco2 (76815)
Gene Description:
calcium binding and coiled-coil domain 2
Transcript:
RefSeq NM_001100177.1 (NON-CURRENT)
Match location:
Position 126 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGATATCATGTGCTATTAC
Hairpin Sequence:
5'-CCGG-GGAGATATCATGTGCTATTAC-CTCGAG-GTAATAGCACATGATATCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGATATCATGTGCTATTACCTCGAGGTAATAGCACATGATATCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGATATCATGTGCTATTACCTCGAGGTAATAGCACATGATATCTCC-3'

Other clones with same target sequence:

(none)