Construct: shRNA TRCN0000253635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001047604.1-255s21c1
DNA Barcode:
CGGGAGTTTGAGGCCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc21b (73668)
Gene Description:
tetratricopeptide repeat domain 21B
Transcript:
RefSeq NM_001047604.1 (NON-CURRENT)
Match location:
Position 255 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGTTTGAGGCCATTAAA
Hairpin Sequence:
5'-CCGG-CGGGAGTTTGAGGCCATTAAA-CTCGAG-TTTAATGGCCTCAAACTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGTTTGAGGCCATTAAACTCGAGTTTAATGGCCTCAAACTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGTTTGAGGCCATTAAACTCGAGTTTAATGGCCTCAAACTCCCG-3'

Other clones with same target sequence:

(none)