Construct: shRNA TRCN0000253654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039752.3-1664s21c1
DNA Barcode:
TTGATGACCTTAACGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC22A10 (387775)
Gene Description:
solute carrier family 22 member 10
Transcript:
RefSeq NM_001039752.3 (NON-CURRENT)
Match location:
Position 1664 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGATGACCTTAACGGTATTT
Hairpin Sequence:
5'-CCGG-TTGATGACCTTAACGGTATTT-CTCGAG-AAATACCGTTAAGGTCATCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGATGACCTTAACGGTATTTCTCGAGAAATACCGTTAAGGTCATCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGATGACCTTAACGGTATTTCTCGAGAAATACCGTTAAGGTCATCAA-3'

Other clones with same target sequence:

(none)