Construct: shRNA TRCN0000253692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020733.1-3562s21c1
DNA Barcode:
GATCTCAGAGGCGGATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HEG1 (57493)
Gene Description:
heart development protein with EGF like domains 1
Transcript:
RefSeq NM_020733.1 (NON-CURRENT)
Match location:
Position 3562 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTCAGAGGCGGATCTTTA
Hairpin Sequence:
5'-CCGG-GATCTCAGAGGCGGATCTTTA-CTCGAG-TAAAGATCCGCCTCTGAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTCAGAGGCGGATCTTTACTCGAGTAAAGATCCGCCTCTGAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTCAGAGGCGGATCTTTACTCGAGTAAAGATCCGCCTCTGAGATC-3'

Other clones with same target sequence:

(none)