Construct: shRNA TRCN0000253708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015562.1-2901s21c1
DNA Barcode:
CCCTATGCTCATTTCGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBXN7 (26043)
Gene Description:
UBX domain protein 7
Transcript:
RefSeq NM_015562.1 (NON-CURRENT)
Match location:
Position 2901 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATGCTCATTTCGTATTT
Hairpin Sequence:
5'-CCGG-CCCTATGCTCATTTCGTATTT-CTCGAG-AAATACGAAATGAGCATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATGCTCATTTCGTATTTCTCGAGAAATACGAAATGAGCATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATGCTCATTTCGTATTTCTCGAGAAATACGAAATGAGCATAGGG-3'

Other clones with same target sequence:

(none)