Construct: shRNA TRCN0000253784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021241.2-2226s21c1
DNA Barcode:
AGCGGCAGAACATCAACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WIZ (58525)
Gene Description:
WIZ zinc finger
Transcript:
RefSeq NM_021241.2 (NON-CURRENT)
Match location:
Position 2226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGCAGAACATCAACAAAT
Hairpin Sequence:
5'-CCGG-AGCGGCAGAACATCAACAAAT-CTCGAG-ATTTGTTGATGTTCTGCCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGCAGAACATCAACAAATCTCGAGATTTGTTGATGTTCTGCCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGCAGAACATCAACAAATCTCGAGATTTGTTGATGTTCTGCCGCT-3'

Other clones with same target sequence:

(none)