Construct: shRNA TRCN0000253847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032223.2-6127s21c1
DNA Barcode:
CACCTCCTAGGATTCAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCNX3 (399909)
Gene Description:
pecanex 3
Transcript:
RefSeq NM_032223.2 (NON-CURRENT)
Match location:
Position 6127 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTCCTAGGATTCAGTAAC
Hairpin Sequence:
5'-CCGG-CACCTCCTAGGATTCAGTAAC-CTCGAG-GTTACTGAATCCTAGGAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTCCTAGGATTCAGTAACCTCGAGGTTACTGAATCCTAGGAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTCCTAGGATTCAGTAACCTCGAGGTTACTGAATCCTAGGAGGTG-3'

Other clones with same target sequence:

(none)