Construct: shRNA TRCN0000253854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030647.1-466s21c1
DNA Barcode:
TGGATTTGATGTCCCTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM7A (80853)
Gene Description:
lysine demethylase 7A
Transcript:
RefSeq NM_030647.1 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATTTGATGTCCCTATTAT
Hairpin Sequence:
5'-CCGG-TGGATTTGATGTCCCTATTAT-CTCGAG-ATAATAGGGACATCAAATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATTTGATGTCCCTATTATCTCGAGATAATAGGGACATCAAATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATTTGATGTCCCTATTATCTCGAGATAATAGGGACATCAAATCCA-3'

Other clones with same target sequence:

  1. TRCN0000238569