Construct: shRNA TRCN0000253978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001184.1-1308s21c1
DNA Barcode:
CCCGGAAGAATTACTGGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Primpol (408022)
Gene Description:
primase and polymerase (DNA-directed)
Transcript:
RefSeq NM_001001184.1 (CURRENT)
Match location:
Position 1308 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGAAGAATTACTGGTTTA
Hairpin Sequence:
5'-CCGG-CCCGGAAGAATTACTGGTTTA-CTCGAG-TAAACCAGTAATTCTTCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGAAGAATTACTGGTTTACTCGAGTAAACCAGTAATTCTTCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGAAGAATTACTGGTTTACTCGAGTAAACCAGTAATTCTTCCGGG-3'

Other clones with same target sequence:

(none)