Construct: shRNA TRCN0000254004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029342.4-222s21c1
DNA Barcode:
TTGCGGAGAACTCACTCTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nhej1 (75570)
Gene Description:
non-homologous end joining factor 1
Transcript:
RefSeq NM_029342.4 (CURRENT)
Match location:
Position 222 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCGGAGAACTCACTCTTAG
Hairpin Sequence:
5'-CCGG-TTGCGGAGAACTCACTCTTAG-CTCGAG-CTAAGAGTGAGTTCTCCGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCGGAGAACTCACTCTTAGCTCGAGCTAAGAGTGAGTTCTCCGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCGGAGAACTCACTCTTAGCTCGAGCTAAGAGTGAGTTCTCCGCAA-3'

Other clones with same target sequence:

(none)