Construct: shRNA TRCN0000254036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001136239.1-2201s21c1
DNA Barcode:
CTGCAAGGAAAGTCATGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM6 (93166)
Gene Description:
PR/SET domain 6
Transcript:
RefSeq NM_001136239.1 (NON-CURRENT)
Match location:
Position 2201 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAAGGAAAGTCATGATTA
Hairpin Sequence:
5'-CCGG-CTGCAAGGAAAGTCATGATTA-CTCGAG-TAATCATGACTTTCCTTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAAGGAAAGTCATGATTACTCGAGTAATCATGACTTTCCTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAAGGAAAGTCATGATTACTCGAGTAATCATGACTTTCCTTGCAG-3'

Other clones with same target sequence:

(none)