Construct: shRNA TRCN0000254069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015123.1-644s21c1
DNA Barcode:
CCGAGTTCTTGACCACGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRMD4B (23150)
Gene Description:
FERM domain containing 4B
Transcript:
RefSeq NM_015123.1 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTTCTTGACCACGATTT
Hairpin Sequence:
5'-CCGG-CCGAGTTCTTGACCACGATTT-CTCGAG-AAATCGTGGTCAAGAACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTTCTTGACCACGATTTCTCGAGAAATCGTGGTCAAGAACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTTCTTGACCACGATTTCTCGAGAAATCGTGGTCAAGAACTCGG-3'

Other clones with same target sequence:

(none)