Construct: shRNA TRCN0000254080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001394.3-220s21c1
DNA Barcode:
GAAGCGGAGAGGAGATTCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJB3 (414061)
Gene Description:
DnaJ heat shock protein family (Hsp40) member B3
Transcript:
RefSeq NM_001001394.3 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCGGAGAGGAGATTCAAG
Hairpin Sequence:
5'-CCGG-GAAGCGGAGAGGAGATTCAAG-CTCGAG-CTTGAATCTCCTCTCCGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCGGAGAGGAGATTCAAGCTCGAGCTTGAATCTCCTCTCCGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCGGAGAGGAGATTCAAGCTCGAGCTTGAATCTCCTCTCCGCTTC-3'

Other clones with same target sequence:

(none)