Construct: shRNA TRCN0000254083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001524.2-702s21c1
DNA Barcode:
GACCTGGCCCTTGTCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TM6SF2 (53345)
Gene Description:
transmembrane 6 superfamily member 2
Transcript:
RefSeq NM_001001524.2 (NON-CURRENT)
Match location:
Position 702 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTGGCCCTTGTCATATAT
Hairpin Sequence:
5'-CCGG-GACCTGGCCCTTGTCATATAT-CTCGAG-ATATATGACAAGGGCCAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTGGCCCTTGTCATATATCTCGAGATATATGACAAGGGCCAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTGGCCCTTGTCATATATCTCGAGATATATGACAAGGGCCAGGTC-3'

Other clones with same target sequence:

(none)