Construct: shRNA TRCN0000254147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027270.1-3193s21c1
DNA Barcode:
ATGTGCCAGTTGCTCACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Exoc1 (69940)
Gene Description:
exocyst complex component 1
Transcript:
RefSeq NM_027270.1 (NON-CURRENT)
Match location:
Position 3193 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGCCAGTTGCTCACAAAT
Hairpin Sequence:
5'-CCGG-ATGTGCCAGTTGCTCACAAAT-CTCGAG-ATTTGTGAGCAACTGGCACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGCCAGTTGCTCACAAATCTCGAGATTTGTGAGCAACTGGCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGCCAGTTGCTCACAAATCTCGAGATTTGTGAGCAACTGGCACAT-3'

Other clones with same target sequence:

(none)