Construct: shRNA TRCN0000254159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178923.3-2337s21c1
DNA Barcode:
AGGAATAAAGGCGGATTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scaf4 (224432)
Gene Description:
SR-related CTD-associated factor 4
Transcript:
RefSeq NM_178923.3 (NON-CURRENT)
Match location:
Position 2337 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAATAAAGGCGGATTATAA
Hairpin Sequence:
5'-CCGG-AGGAATAAAGGCGGATTATAA-CTCGAG-TTATAATCCGCCTTTATTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAATAAAGGCGGATTATAACTCGAGTTATAATCCGCCTTTATTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAATAAAGGCGGATTATAACTCGAGTTATAATCCGCCTTTATTCCT-3'

Other clones with same target sequence:

(none)