Construct: shRNA TRCN0000254229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026922.1-1297s21c1
DNA Barcode:
ACTCTGACAGCCAACGAAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2c2 (69047)
Gene Description:
ATPase, Ca++ transporting, type 2C, member 2
Transcript:
RefSeq NM_026922.1 (CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCTGACAGCCAACGAAATG
Hairpin Sequence:
5'-CCGG-ACTCTGACAGCCAACGAAATG-CTCGAG-CATTTCGTTGGCTGTCAGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCTGACAGCCAACGAAATGCTCGAGCATTTCGTTGGCTGTCAGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCTGACAGCCAACGAAATGCTCGAGCATTTCGTTGGCTGTCAGAGT-3'

Other clones with same target sequence:

(none)