Construct: shRNA TRCN0000254312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025153.2-4102s21c1
DNA Barcode:
CTTTGCCATCACCCGCTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP10B (23120)
Gene Description:
ATPase phospholipid transporting 10B (putative)
Transcript:
RefSeq NM_025153.2 (NON-CURRENT)
Match location:
Position 4102 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCCATCACCCGCTTTAA
Hairpin Sequence:
5'-CCGG-CTTTGCCATCACCCGCTTTAA-CTCGAG-TTAAAGCGGGTGATGGCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCCATCACCCGCTTTAACTCGAGTTAAAGCGGGTGATGGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCCATCACCCGCTTTAACTCGAGTTAAAGCGGGTGATGGCAAAG-3'

Other clones with same target sequence:

(none)