Construct: shRNA TRCN0000254335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024562.1-339s21c1
DNA Barcode:
GATCCGCCTTGCAGCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANGO6 (79613)
Gene Description:
transport and golgi organization 6 homolog
Transcript:
RefSeq NM_024562.1 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCGCCTTGCAGCTAATTT
Hairpin Sequence:
5'-CCGG-GATCCGCCTTGCAGCTAATTT-CTCGAG-AAATTAGCTGCAAGGCGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCGCCTTGCAGCTAATTTCTCGAGAAATTAGCTGCAAGGCGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCGCCTTGCAGCTAATTTCTCGAGAAATTAGCTGCAAGGCGGATC-3'

Other clones with same target sequence:

(none)