Construct: shRNA TRCN0000254374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175550.3-3062s21c1
DNA Barcode:
TCCATGAACTTACCATTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap4e1 (108011)
Gene Description:
adaptor-related protein complex AP-4, epsilon 1
Transcript:
RefSeq NM_175550.3 (CURRENT)
Match location:
Position 3062 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATGAACTTACCATTATTA
Hairpin Sequence:
5'-CCGG-TCCATGAACTTACCATTATTA-CTCGAG-TAATAATGGTAAGTTCATGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATGAACTTACCATTATTACTCGAGTAATAATGGTAAGTTCATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATGAACTTACCATTATTACTCGAGTAATAATGGTAAGTTCATGGA-3'

Other clones with same target sequence:

(none)