Construct: shRNA TRCN0000254405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099296.1-647s21c1
DNA Barcode:
GTCGGAGAAAGAGCGATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grrp1 (72690)
Gene Description:
glycine/arginine rich protein 1
Transcript:
RefSeq NM_001099296.1 (NON-CURRENT)
Match location:
Position 647 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGAGAAAGAGCGATTCTT
Hairpin Sequence:
5'-CCGG-GTCGGAGAAAGAGCGATTCTT-CTCGAG-AAGAATCGCTCTTTCTCCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGAGAAAGAGCGATTCTTCTCGAGAAGAATCGCTCTTTCTCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGAGAAAGAGCGATTCTTCTCGAGAAGAATCGCTCTTTCTCCGAC-3'

Other clones with same target sequence:

(none)