Construct: shRNA TRCN0000254474

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001137671.1-1230s21c1
DNA Barcode:
TCTTATATGGTGCTGATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEC (388468)
Gene Description:
POTE ankyrin domain family member C
Transcript:
RefSeq NM_001137671.1 (CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254474)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTATATGGTGCTGATATTG
Hairpin Sequence:
5'-CCGG-TCTTATATGGTGCTGATATTG-CTCGAG-CAATATCAGCACCATATAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTATATGGTGCTGATATTGCTCGAGCAATATCAGCACCATATAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTATATGGTGCTGATATTGCTCGAGCAATATCAGCACCATATAAGA-3'

Other clones with same target sequence:

(none)