Construct: shRNA TRCN0000254489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183296.1-308s21c1
DNA Barcode:
CTGAGTGAACTGCTGATCTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap16-3 (71369)
Gene Description:
keratin associated protein 16-3
Transcript:
RefSeq NM_183296.1 (CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGTGAACTGCTGATCTTC
Hairpin Sequence:
5'-CCGG-CTGAGTGAACTGCTGATCTTC-CTCGAG-GAAGATCAGCAGTTCACTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGTGAACTGCTGATCTTCCTCGAGGAAGATCAGCAGTTCACTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGTGAACTGCTGATCTTCCTCGAGGAAGATCAGCAGTTCACTCAG-3'

Other clones with same target sequence:

(none)