Construct: shRNA TRCN0000254507

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113379.1-3491s21c1
DNA Barcode:
GTCAGCCAGAGCTTCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc32 (434215)
Gene Description:
leucine rich repeat containing 32
Transcript:
RefSeq NM_001113379.1 (CURRENT)
Match location:
Position 3491 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254507)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGCCAGAGCTTCCGTAAT
Hairpin Sequence:
5'-CCGG-GTCAGCCAGAGCTTCCGTAAT-CTCGAG-ATTACGGAAGCTCTGGCTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGCCAGAGCTTCCGTAATCTCGAGATTACGGAAGCTCTGGCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGCCAGAGCTTCCGTAATCTCGAGATTACGGAAGCTCTGGCTGAC-3'

Other clones with same target sequence:

(none)