Construct: shRNA TRCN0000254581
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001033382.2-4014s21c1
- DNA Barcode:
- TAGGAACGCAATGGATATTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cacna2d4 (319734)
- Gene Description:
- calcium channel, voltage-dependent, alpha 2/delta subunit 4
- Transcript:
- RefSeq NM_001033382.2 (CURRENT)
- Match location:
- Position 4014 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000254581)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TAGGAACGCAATGGATATTAA
- Hairpin Sequence:
-
5'-CCGG-TAGGAACGCAATGGATATTAA-CTCGAG-TTAATATCCATTGCGTTCCTA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTAGGAACGCAATGGATATTAACTCGAGTTAATATCCATTGCGTTCCTATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATAGGAACGCAATGGATATTAACTCGAGTTAATATCCATTGCGTTCCTA-3'
Other clones with same target sequence:
(none)
