Construct: shRNA TRCN0000254581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033382.2-4014s21c1
DNA Barcode:
TAGGAACGCAATGGATATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacna2d4 (319734)
Gene Description:
calcium channel, voltage-dependent, alpha 2/delta subunit 4
Transcript:
RefSeq NM_001033382.2 (CURRENT)
Match location:
Position 4014 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGAACGCAATGGATATTAA
Hairpin Sequence:
5'-CCGG-TAGGAACGCAATGGATATTAA-CTCGAG-TTAATATCCATTGCGTTCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGAACGCAATGGATATTAACTCGAGTTAATATCCATTGCGTTCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGAACGCAATGGATATTAACTCGAGTTAATATCCATTGCGTTCCTA-3'

Other clones with same target sequence:

(none)