Construct: shRNA TRCN0000254645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039555.2-837s21c1
DNA Barcode:
TCCTCGGGACTTCGTTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2c68 (433247)
Gene Description:
cytochrome P450, family 2, subfamily c, polypeptide 68
Transcript:
RefSeq NM_001039555.2 (CURRENT)
Match location:
Position 837 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTCGGGACTTCGTTGATTA
Hairpin Sequence:
5'-CCGG-TCCTCGGGACTTCGTTGATTA-CTCGAG-TAATCAACGAAGTCCCGAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTCGGGACTTCGTTGATTACTCGAGTAATCAACGAAGTCCCGAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTCGGGACTTCGTTGATTACTCGAGTAATCAACGAAGTCCCGAGGA-3'

Other clones with same target sequence:

(none)