Construct: shRNA TRCN0000254689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100185.1-275s21c1
DNA Barcode:
ATGCACGTGTCGTGATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4a30b (435802)
Gene Description:
cytochrome P450, family 4, subfamily a, polypeptide 30b
Transcript:
RefSeq NM_001100185.1 (CURRENT)
Match location:
Position 275 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCACGTGTCGTGATCTATG
Hairpin Sequence:
5'-CCGG-ATGCACGTGTCGTGATCTATG-CTCGAG-CATAGATCACGACACGTGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCACGTGTCGTGATCTATGCTCGAGCATAGATCACGACACGTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCACGTGTCGTGATCTATGCTCGAGCATAGATCACGACACGTGCAT-3'

Other clones with same target sequence:

(none)