Construct: shRNA TRCN0000254864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014291.3-262s21c1
DNA Barcode:
ACCTCCACCATGCCAGGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2G (6706)
Gene Description:
small proline rich protein 2G
Transcript:
RefSeq NM_001014291.3 (NON-CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCCACCATGCCAGGATAA
Hairpin Sequence:
5'-CCGG-ACCTCCACCATGCCAGGATAA-CTCGAG-TTATCCTGGCATGGTGGAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCCACCATGCCAGGATAACTCGAGTTATCCTGGCATGGTGGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCCACCATGCCAGGATAACTCGAGTTATCCTGGCATGGTGGAGGT-3'

Other clones with same target sequence:

(none)