Construct: shRNA TRCN0000254867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014291.3-284s21c1
DNA Barcode:
TGCCCTCCTGTGCAACCATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2G (6706)
Gene Description:
small proline rich protein 2G
Transcript:
RefSeq NM_001014291.3 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCCTCCTGTGCAACCATAC
Hairpin Sequence:
5'-CCGG-TGCCCTCCTGTGCAACCATAC-CTCGAG-GTATGGTTGCACAGGAGGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCCTCCTGTGCAACCATACCTCGAGGTATGGTTGCACAGGAGGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCCTCCTGTGCAACCATACCTCGAGGTATGGTTGCACAGGAGGGCA-3'

Other clones with same target sequence:

(none)