Construct: shRNA TRCN0000254936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024931.2-2624s21c1
DNA Barcode:
GGATAGGTGGAGTAGGGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbfox3 (52897)
Gene Description:
RNA binding protein, fox-1 homolog (C. elegans) 3
Transcript:
RefSeq NM_001024931.2 (CURRENT)
Match location:
Position 2624 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATAGGTGGAGTAGGGTTAA
Hairpin Sequence:
5'-CCGG-GGATAGGTGGAGTAGGGTTAA-CTCGAG-TTAACCCTACTCCACCTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATAGGTGGAGTAGGGTTAACTCGAGTTAACCCTACTCCACCTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATAGGTGGAGTAGGGTTAACTCGAGTTAACCCTACTCCACCTATCC-3'

Other clones with same target sequence:

(none)