Construct: shRNA TRCN0000254983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024717.1-693s21c1
DNA Barcode:
TGCCTCCAGACACGATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gal3st3 (545276)
Gene Description:
galactose-3-O-sulfotransferase 3
Transcript:
RefSeq NM_001024717.1 (NON-CURRENT)
Match location:
Position 693 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTCCAGACACGATCTATG
Hairpin Sequence:
5'-CCGG-TGCCTCCAGACACGATCTATG-CTCGAG-CATAGATCGTGTCTGGAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTCCAGACACGATCTATGCTCGAGCATAGATCGTGTCTGGAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTCCAGACACGATCTATGCTCGAGCATAGATCGTGTCTGGAGGCA-3'

Other clones with same target sequence:

(none)