Construct: shRNA TRCN0000254985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024717.1-890s21c1
DNA Barcode:
TACGACCTTGGAGGCGACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gal3st3 (545276)
Gene Description:
galactose-3-O-sulfotransferase 3
Transcript:
RefSeq NM_001024717.1 (NON-CURRENT)
Match location:
Position 890 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGACCTTGGAGGCGACAAT
Hairpin Sequence:
5'-CCGG-TACGACCTTGGAGGCGACAAT-CTCGAG-ATTGTCGCCTCCAAGGTCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGACCTTGGAGGCGACAATCTCGAGATTGTCGCCTCCAAGGTCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGACCTTGGAGGCGACAATCTCGAGATTGTCGCCTCCAAGGTCGTA-3'

Other clones with same target sequence:

(none)