Construct: shRNA TRCN0000255017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033500.3-1518s21c1
DNA Barcode:
CCCTAGAGAAATACCAATAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr72 (546144)
Gene Description:
WD repeat domain 72
Transcript:
RefSeq NM_001033500.3 (CURRENT)
Match location:
Position 1518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAGAGAAATACCAATAAC
Hairpin Sequence:
5'-CCGG-CCCTAGAGAAATACCAATAAC-CTCGAG-GTTATTGGTATTTCTCTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAGAGAAATACCAATAACCTCGAGGTTATTGGTATTTCTCTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAGAGAAATACCAATAACCTCGAGGTTATTGGTATTTCTCTAGGG-3'

Other clones with same target sequence:

(none)