Construct: shRNA TRCN0000255032

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025575.1-932s21c1
DNA Barcode:
GTAAACGTGGATATCAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfhr2 (545366)
Gene Description:
complement factor H-related 2
Transcript:
RefSeq NM_001025575.1 (NON-CURRENT)
Match location:
Position 932 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255032)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAACGTGGATATCAGAAAG
Hairpin Sequence:
5'-CCGG-GTAAACGTGGATATCAGAAAG-CTCGAG-CTTTCTGATATCCACGTTTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAACGTGGATATCAGAAAGCTCGAGCTTTCTGATATCCACGTTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAACGTGGATATCAGAAAGCTCGAGCTTTCTGATATCCACGTTTAC-3'

Other clones with same target sequence:

(none)