Construct: shRNA TRCN0000255081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037155.1-1132s21c1
DNA Barcode:
GACGTCTCATTGCCCTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsn2 (406236)
Gene Description:
hereditary sensory neuropathy, type II
Transcript:
RefSeq NM_001037155.1 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGTCTCATTGCCCTTTATT
Hairpin Sequence:
5'-CCGG-GACGTCTCATTGCCCTTTATT-CTCGAG-AATAAAGGGCAATGAGACGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGTCTCATTGCCCTTTATTCTCGAGAATAAAGGGCAATGAGACGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGTCTCATTGCCCTTTATTCTCGAGAATAAAGGGCAATGAGACGTC-3'

Other clones with same target sequence:

(none)