Construct: shRNA TRCN0000255098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039234.1-795s21c1
DNA Barcode:
TTGCTGCATTTAGATTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nlrp1c-ps (627984)
Gene Description:
NLR family, pyrin domain containing 1C, pseudogene
Transcript:
RefSeq NM_001039234.1 (NON-CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCTGCATTTAGATTGATTA
Hairpin Sequence:
5'-CCGG-TTGCTGCATTTAGATTGATTA-CTCGAG-TAATCAATCTAAATGCAGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCTGCATTTAGATTGATTACTCGAGTAATCAATCTAAATGCAGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCTGCATTTAGATTGATTACTCGAGTAATCAATCTAAATGCAGCAA-3'

Other clones with same target sequence:

(none)