Construct: shRNA TRCN0000255112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033191.2-465s21c1
DNA Barcode:
TTACGCCATGAAGCGGGATCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naa11 (97243)
Gene Description:
N(alpha)-acetyltransferase 11, NatA catalytic subunit
Transcript:
RefSeq NM_001033191.2 (CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACGCCATGAAGCGGGATCT
Hairpin Sequence:
5'-CCGG-TTACGCCATGAAGCGGGATCT-CTCGAG-AGATCCCGCTTCATGGCGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACGCCATGAAGCGGGATCTCTCGAGAGATCCCGCTTCATGGCGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACGCCATGAAGCGGGATCTCTCGAGAGATCCCGCTTCATGGCGTAA-3'

Other clones with same target sequence:

(none)